Ultrasound‐guided puncture of newborn SD rat cortex, hippocampus, lateral ventricle, and striatum

Since the brain structure of neonatal rats was not fully formed during first 4 days, it cannot be detected using ultrasound. The objective this study to investigate use ultrasound detect changes in normal coronal and determine puncture depth location cortex, hippocampus, lateral ventricle, striatum newborn Sprague–Dawley (SD) 5−15 days. animal placed a prone position. specific positions were measured under Then, SD punctured with stereotaxic instrument, dye injected. Finally, brains taken make frozen sections observe results. By ultrasound, image rat can obtained cortex (8 days: 1.02 ± 0.12, 10 0.08, 13 1.43 0.05), hippocampus 2.63 0.07, 2.77 0.14, 2.82 0.09), ventricle 2.08 0.04, 2.26 0.03, 2.40 0.06), corpus 4.57 0.09, 4.94 0.31, 5.13 0.10) accurately measured. changed age rats. Ultrasound technology only clarify characteristics 5−15-d but also guide injection structure. results laid foundation for future experimental models neurological diseases.